Applications:

• Simulating Mechanical and Biological Stresses: The bioreactor applies cyclic mechanical pressures and fluid flow to mimic the chewing forces and salivary flow in the mouth. This helps assess how dental adhesives and restorations perform under realistic loads and conditions.
• Biofilm Formation and Challenge Testing: It facilitates the growth and maintenance of mature bacterial biofilms, allowing researchers to evaluate the effects of biofilm-related challenges like bacterial acids and enzymes on the integrity of dental adhesives and the tooth-restoration interface.
• Assessment of Bond Durability and Marginal Integrity: It helps determine how different materials withstand mechanical wear and bacterial stress over time, facilitating evaluation of marginal gap formation, bond strength, and collagen reinforcement under conditions that closely resemble the demanding oral environment.

"The ODEME bioreactor was idealized to address a gap in testing in biomaterials. Its unique features allow for simulated restorations to be challenged by mechanical and bacterial loads simultaneously, mimicking conditions in the oral environment. The current model allows for precise temperature control, and the design of the chambers allows for culture medium and specialty gas flow around the specimens. Depending on the culture medium and other conditions, several different microorganism species can be tested. So far, we have used Streptococcus mutans and Staphylococcus aureus, and are planning on using more complex microcosm models in the near future. We have also adapted the set up to test titanium species bonded to bone, in a study testing the efficiency of bone cements. This is truly a versatile and physiologically relevant set up." - Carmem S. Pfeifer, DDS, PhD – Oregon Health & Science University (OHSU)

Technical features:

• •       Three detachable and autoclavable test chambers;
• •       Thermal control in the chambers (20-40ºC);
• •       Electro-pneumatic charging system with up to 4 test routines;
• •       Pneumatics imported from Japan;
• •       Automatic pressure adjustment;
• •       Inlet pressure regulator with line filter included;
• •       Colorful and intuitive touchscreen panel;
• •       Intelligent maintenance system included in the machine;
• •       Stainless steel actuator with spherical tip;
• •       Capacity of each chamber: 1 (one) specimen;
• •       Fluid input and output connectors;
• •       Gas inlet and outlet connector;
• •       Control by industrial PLC;
• •       Adjustment of test routines with control of cycles, frequency and time;
• •       Automatic shutdown;
• •       Protections against power outages with automatic test return;
• •       Minimum load: 0.9 kgf
• •       Maximum load: 20 kgf
• •       Test frequency: 0.5 to 2.0 Hz
• •       Emergency button (NR 10)
• •       NR12 protections
• •       Supply voltage: selector 110 or 220V

Included:

• Fluid injection and removal system in the chambers (2 pumps with 3 channels each with touch screen and microflow control - included); 
• Three extra cameras 
• Digital calibration load device